2.8. RNA Extraction, RT-PCR, and Real-Time RT-PCR

Total RNA was extracted using TIANGEN RNA Pre Micro kit according to the manufacturer's instruction. Reverse transcription was performed using oligo (dT) priming and M-MLV reverse transcriptase (Promega) following the manufacturer's instructions. PCR primers were listed in Table 1. PCR products were separated by electrophoresis on 1.5% agarose gels. Real-time RT-PCR were performed on ABI 7500 sequence detection system and analysed with ABI 7500 software.

PCR primer sequences and product size.