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HEK293T cells were plated at 60% confluence in a 12-well and 16 h later transfected with 1 µg of editor expression vector and 0.5 µg LRT2B plasmid containing gRNA of interest in 100 µl DMEM using a 1:3 DNA:PEI ratio. Cells were washed 12 h later in complete media. Cells were assayed and collected 2–7 days post-transfection. For PE3, 0.5 µg of nicking gRNA in LRT2B was transfected 24 h after the primary transfection.
Copyright and License information: ©2020 The Author(s) 2020. Published by Oxford University Press on behalf of Nucleic Acids Research.
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact moc.puo@snoissimrep.slanruoj
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