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Immunofluorescence and staining for γH2AX
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Immunofluorescence staining and counting of γH2AX nuclear foci was performed as previously described [45]. Slides were mounted and images captured using Olympus FSX100 fluorescent microscope. For each treatment condition, foci were determined in at least 150 cells. Image-J (NIH) software was used to analyze the mean number of foci with combined area of γH2AX foci per nucleus.
Copyright and License information: ©2016 Maachani et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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