SARS-CoV-2 isolate (HIAE-02: SARS-CoV-2/SP02/human/2020/BRA (GenBank accession number MT126808.1) was used in this work. The virus was grown in Vero cells (Monkey African Green kidney cell line – ATCC CCL-81) in the Laboratory of Molecular Virology, at Federal University of Rio de Janeiro, Brazil. Vero cells were maintained in DMEM supplemented with 5% fetal bovine serum (FBS; GIBCO) at 37 °C and 5% CO2. All work involving infectious SARS-CoV2 was performed in a biosafety level (BSL)-3 containment laboratory.
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