Cells and virus

LC Lucio Ayres Caldas
FC Fabiana Avila Carneiro
LH Luiza Mendonça Higa
FM Fábio Luiz Monteiro
GS Gustavo Peixoto da Silva
LC Luciana Jesus da Costa
ED Edison Luiz Durigon
AT Amilcar Tanuri
WS Wanderley de Souza
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SARS-CoV-2 isolate (HIAE-02: SARS-CoV-2/SP02/human/2020/BRA (GenBank accession number MT126808.1) was used in this work. The virus was grown in Vero cells (Monkey African Green kidney cell line – ATCC CCL-81) in the Laboratory of Molecular Virology, at Federal University of Rio de Janeiro, Brazil. Vero cells were maintained in DMEM supplemented with 5% fetal bovine serum (FBS; GIBCO) at 37 °C and 5% CO2. All work involving infectious SARS-CoV2 was performed in a biosafety level (BSL)-3 containment laboratory.

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