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Cell migration was examined by transwell experiment. Transfected DU145 and LNCap cells cultured with serum-free medium and then seeded them into upper chamber. And the lower chamber containing the complete medium. The ability to migrate could be demonstrated by observing the number of cells stained by 0.1% crystal violet through the membrane. The invasion assay was added with the matrigel (BD Biosciences, San Jose, CA, USA) in upper chamber and the other steps were same with migration assay. All the experiments were carried out under a 100× optical microscope.
Copyright and License information: ©2020 Zhang et al.
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