2.12. Human Skin Explant Culture

Human skin explants (HSE) were obtained from breast or abdominal tissue samples of healthy adult donors (18–60 years old) undergoing plastic surgery. After subcutaneous fat deletion, 12 mm punch biopsies were rinsed with phosphate buffered saline (PBS) and cultured in Dulbecco’s Modified Eagle Medium (DMEM) containing 100 U/mL penicillin, 100 µg/mL streptomycin, 25 μg amphotericin B (Sigma-Aldrich, Germany), 1% L-glutamine (Sigma-Aldrich, Germany) and 10% FBS at 37 °C in 5% CO₂ humidified air into 6-well plates [28]. The next day, 20 μL of SLN-P-CA were topically applied for 24 h on HSEs cultured in fresh medium.