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Cell survival was evaluated using a standard colony forming assay [21]. To determine clonogenic survival under hypoxic conditions, cells were exposed to 0.5% oxygenated conditions in a 1000 in vivo hypoxic chamber (BioTrace, Bracknell, UK) for 24 h prior to hypoxic irradiation. The cells were returned to an aerobic incubator following irradiation and during colony formation. Colonies were counted using the ColCount instrument (Oxford Optronix Ltd, Oxford, UK).
Copyright and License information: ©2017 The Authors
This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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