Postnatal MRI and histological reference series

An interleaved anatomical atlas of magnetic resonance imaging (MRI), histology, and block face photographs was created from four male rhesus macaques at 2 weeks, 3, 12 and 48 months after birth as described in the “Reference Series” white paper. In addition, ISH data were generated serially across complete hemispheres of three post-pubertal adult (48 months) male specimens. Genes were selected to mark specific cell populations and cortical areas and to represent gene families important to neural function and associated with neuropsychiatric disease and brain evolution. Several histological stains (Nissl, acetylcholinesterase and SMI-32) of complete hemispheres were combined with ISH data to provide an unprecedented cellular resolution view of gene expression in the whole post-pubertal adult brain. High-throughput colorimetric ISH methods are described in detail elsewhere⁴⁹ and in the “In Situ Hybridization” white paper.