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2.4. Imaging

MY Minjung Yoon
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Immunolabeling of the sample tissues was observed using a Leica DM2500 fluorescent microscope (Leica, Wetzlar, Germany). The microscope was equipped with an EL 6000 external light source (Leica). Green and red fluorescent expressions were observed using a dual-emission fluorescein-5-isothiocyanate (FITC)/ tetramethyl rhodamine isothiocyanate (TRITC) filter. Cells showing green or red fluorescence were considered positive for either PGP9.5 or DAZL, whereas cells with no fluorescence were considered negative. The immunolabeled images were captured using a Leica DFC 450 C digital camera.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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