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2.13. Determination of Transepithelial Electrical Resistance
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Caco-2 cells (1 × 105 cells/well) were seeded in Transwell inserts (membrane area 0.33 cm2, pore size 0.4 μm) placed in 24-well plates. Transepithelial electrical resistance (TEER) was monitored daily using an EVOM voltohmmeter with STX2 electrodes (World Precision Instruments). When the resistance approached 1000 Ω cm2, cells were pretreated with or without GPA for 6 h followed by treatment with LPS. TEER was measured hourly during 6 h LPS treatment.
Copyright and License information: ©2020 Zhao Deng et al.
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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