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Doxycycline hyclate (Sigma Aldrich,) stock solutions were made at 40mg/mL or 10mg/mL in water. A conversion factor of 93.76% was used to account for the hyclate. Samples were aliquoted into Eppendorf tubes and stored at −20°C protected from light. Stock solutions were diluted to final concentrations with medium.
Copyright and License information: ©2016 Alexander et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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