Morris Water Maze Test

The standard Morris water maze procedure was used with minor modifications [21]. Briefly, the rats (n = 10/group) received hcy and simultaneous Lir injection for 14 days and were trained to find a hidden platform in water maze for five consecutive days, with four trials/day at 30-min intervals. The rats were allowed to search for the platform for up to 60 s, after which they were guided to the platform and allowed to stay on it for 20 s. In each trial, the swimming pathway and latency to locate the platform were recorded using a video tracking system (Zhenghua, Anhui, China). In the probe trials measured on day 7, rats were allowed to swim for 60 s after the hidden platform had been removed. The number of times the platform was crossed, the time to first reach the former location of the platform (latency), and the swimming path were recorded.