Apoptosis detection by DAPI staining

4,6-Diamidino-2-phenylindole (DAPI) staining was performed to detect chromatin fragmentation according to previous studies (Karami et al., 2013[19]; Armat et al., 2016[1]). This technique is based on fluorescence creation following the binding of DAPI to DNA molecules. MiR-145 and control cells were cultured in 6-well plates. After 24 h, the cells were fixed using 4 % paraformaldehyde for 15 min and permeabilized using 0.1 % Triton-X-100 for 10 min at RT. DAPI with a final concentration of 1:500 (in PBS) was utilized for staining. Depending on the morphological characteristics, the cells were recognized as normal or apoptotic. Cytation 5 cell imaging system was used to perform imaging.