Improve Research Reproducibility A Bio-protocol resource
Concise Method
tooltip

Characterization of isolated EV samples

MT Mette Elise Tunset
HH Hanne Haslene-Hox
TB Tim Van Den Bossche
AV Arne Einar Vaaler
ES Einar Sulheim
DK Daniel Kondziella
request Request a Protocol
ask Ask a question
Favorite

The protein concentration in isolated EV samples was determined by Qubit Quant-IT Protein Assay Kit (Thermo Fisher Scientific, cat. no. Q33211) on a Qubit Fluorometer 2.0. EVs were analyzed for size and concentration using Nanoparticle Tracking Analysis (NTA, Nanosight LM10, Malvern Panalytical Ltd, Malvern, UK). EVs were diluted 100-fold in sterile PBS and three one-minute movies were recorded on the NTA (detection threshold 4, auto blur size, max jump distance).

Copyright and License information:  ©2020 Tunset et al.
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A