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Breast cancer cells were seeded 1×103 cells in a 6-well tissue culture plate; after 24 hours they were treated with various concentrations of CTS and allowed to grow for 6 days (MCF-7) or 10 days (MDA-MB-231) to form colonies. Cells were stained by 0.1% crystal violet. The total number of colonies formed on each well were analyzed.
Copyright and License information: ©2020 Zhou et al.
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