Cell viability assay

Hep3B or HCCLM3 cells were trypsinzed and seeded into 96-well plates at a density of approximately 3 × 10³ cells per well. After 24 h, adherent cells were treated with platelet lysates. After 24 h, 48 h and 72 h incubation, MTT reagent was added to the cells (0.5 mg/ml), and cells were then incubated for 4 h at 37°C. The cells media were removed and 150 μl DMSO were added to each well followed by gentle shaking of the plates to dissolve the formazan crystals. The optical density (OD) was then measured using an automated ELISA plate reader at 570 nm.