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Antioxidant response element luciferase reporter assay

YL Yang Liu
YP Ying Pang
BZ Boqun Zhu
OU Ondrej Uher
VC Veronika Caisova
TH Thanh-Truc Huynh
DT David Taieb
KV Katerina Hadrava Vanova
HG Hans Kumar Ghayee
JN Jiri Neuzil
ML Mark Levine
CY Chunzhang Yang
KP Karel Pacak
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Nrf2-driven transcriptional activation was quantified as antioxidant response element (ARE) transcriptional activity using a dual-luciferase reporter system (28, 29). pGL3.47-ARE-Luc plasmid (900 ng) and 100 ng pRL-TK plasmid (Promega) were cotransfected into 8,000 cells using Lipofectamine 3000 (Thermo Fisher Scientific). The firefly (ARE activity) and Renilla (internal control) luminescence signal were assessed using a Polarstar Optima Plate Reader (BMG Labtech).

Copyright and License information:  ©2020

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