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Serum MMP determination

QP Qunyan Pan
TY Tao Yuan
QD Qian Ding
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For all subjects, 6 mL venous blood was collected from the elbow vein under fasting conditions before and after the operation, respectively. Blood samples in the control group were collected after ultrasound contrast examination. The blood samples were placed at room temperature for 30 minutes, and then subjected to centrifugation at 2000 × g for 15 minutes. The serum was harvested and serum levels of MMP-3 and MMP-9 were determined using enzyme-linked immunosorbent assay (ELISA) kits (Boster Bioengineering, Wuhan, Hubei, China).

Copyright and License information: The Author(s) ©2020
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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