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2.2. Carrageenan-Induced Paw Edema
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After anesthesia with 5.0% isoflurane in 100% O2 rats were subjected to a subplantar injection of CAR (0.1 mL/rat of a 1% suspension in saline) with a 27-gauge needle into the right hind paw, as described previously by Morris and Britti [40,41]. The animals were sacrificed after 6 h post CAR-injection by isoflurane overdose. All analyses were performed in a blinded manner of experimental groups [42].
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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