Quantitative analysis of transfection efficiency by flow cytometry

HM Hanieh Moradian
AL Andreas Lendlein
MG Manfred Gossen
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Cells were harvested at previously specified time points by TrypLE Select (Gibco, Germany) according to manufacturer’s instruction. Upon washing with cold flow cytometry washing solution (PBS pH 7.2, BSA, EDTA), cells were analyzed with a MACSQuant VYB® flow cytometer (Miltenyi Biotec, Germany). All flow cytometric data were analyzed with FlowJo software V10.

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