2.10. Statistics

Graphpad Prism (v 8.4.2) was used to plot all the graphs and to determine the statistical significance. For a two-group comparison, an unpaired t-test with Welch’s correction was used to determine the differences between the groups (Figures 2c,e,f, 5b,d and S1a,c,d), unless all samples were matched in which case a paired and parametric t-test was employed (Figures 2b and S1b). For multiple comparisons, ordinary one-way ANOVA test with Dunnett’s correction was used to determine the differences between the groups as compared to the control (Figures 3d, 4c,d and 5d). For 2-variable multiple comparisons, two-way ANOVA with Sidak’s correction was used and the predicted mean difference with adjusted p-value reported (Figure 4e). Error bars represent either standard deviation (S.D.) across multiple measurements, or standard error of the mean (S.E.M.) across independent experiments.