RNA extraction and cDNA synthesis

BM Bruno Paes de Melo
IL Isabela Tristan Lourenço-Tessutti
JP Joaquin Felipe Roca Paixão
DN Daniel David Noriega
MS Maria Cristina Mattar Silva
JA Janice de Almeida-Engler
EF Elizabeth Pacheco Batista Fontes
MG Maria Fatima Grossi-de-Sa
request Request a Protocol
ask Ask a question
Favorite

Total RNA was extracted from 100 to 150 mg of leaves using TRIzol (Invitrogen, Carlsbad, CA-USA) according to the manufacturer’s recommendations. The RNA was quantified spectrophotometrically using a NanoDrop instrument (Thermo Fisher, Waltham, Massachusetts, USA), and its quality and integrity were evaluated by electrophoresis on a 1% (w/v) agarose gel. A total of 1 µg of RNA was used to perform cDNA synthesis according to the MMLV reverse transcriptase (Invitrogen, Carlsbad, CA-USA) protocol.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A