Total RNA was extracted from 100 to 150 mg of leaves using TRIzol (Invitrogen, Carlsbad, CA-USA) according to the manufacturer’s recommendations. The RNA was quantified spectrophotometrically using a NanoDrop instrument (Thermo Fisher, Waltham, Massachusetts, USA), and its quality and integrity were evaluated by electrophoresis on a 1% (w/v) agarose gel. A total of 1 µg of RNA was used to perform cDNA synthesis according to the MMLV reverse transcriptase (Invitrogen, Carlsbad, CA-USA) protocol.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.