Cell lines and cell culture

The A2780 human ovarian cancer cell line was originally established from the tumor tissue of an untreated patient, and the cells grew as a monolayer and in suspension in spinner cultures. The SKOV3 cell line was originally isolated from the ascites of patients with ovarian tumors, and tumorigenesis in nude mice resulted in a moderately differentiated adenocarcinoma consistent with carcinoma in situ of the ovary. The above-mentioned cells were cultured in RPMI-1640 medium and McCoy's 5A medium, respectively. 293T cells, which could continuously express SV40 antigen, was used in the transfection experiments with a high transfection efficiency, and was cultured in DMEM. The A2780 cell line was originally purchased from the European Collection of Authenticated Cell Cultures (ECACC; Cat. no. 93112519). The SKOV3 and 293T cells were purchased from the American Type Culture Collection (ATCC; Cat. nos. HTB-77 and CRL-11268, respectively). All the culture media were supplemented with 10% fetal bovine serum (FBS). All these cells were cultured in a humidified incubator under standard culture conditions (37°C, 5% CO₂).