2.7. DPPH Assay for Evaluating the In Vitro Radical Scavenging Activity

The DPPH assay to determine the antioxidant activity in vitro was performed by a standard method with slight modifications [27]. The DPPH solution (0.0125 mM in MeOH, 45 μL) was added to 15 μL of the OMN and OMU EVOO extracts at different concentrations (1.0 and 50.0 µg/mL). The reaction for scavenging the DPPH radicals was performed in the dark at room temperature (RT) and the absorbance was measured at 520 nm after 30 min incubation.