Senescence-associated β-galactosidase staining was carried out using a commercial kit (Senescence β-Galactosidase Staining Kit, Cell Signaling Technology, Danvers, MA, USA). Cultured MKTPCs were seeded onto cover slips, and the staining was implemented according to manufacturer’s instructions. A Zeiss Axiovert microscope (Zeiss GmbH, Oberkochen, Germany) was used for the examination.
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