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von Frey test for tactile allodynia

AE Andrew L. Eagle
CM Claire E. Manning
EW Elizabeth S. Williams
RB Ryan M. Bastle
PG Paula A. Gajewski
AG Amber Garrison
AW Alexis J. Wirtz
SA Seda Akguen
KB Katie Brandel-Ankrapp
WE Wilson Endege
FB Frederick M. Boyce
YO Yoshinori N. Ohnishi
MM Michelle Mazei-Robison
IM Ian Maze
RN Rachel L. Neve
AR Alfred J. Robison
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Tactile allodynia was assessed using calibrated von Frey filaments as previously described50. Mice were habituated to clear plexiglass containers over a mesh floor for 1 h. The next day mice were placed back into the containers for 30 min prior to testing. A series of von Frey filaments were applied to the plantar surface of the hindpaw with sufficient force to bend the filaments for at least 6 s. A paw withdrawal or rapid flinch response was recorded. In the absence of this response, a filament of the next greater force was applied. If a response occurred, the next lower filament was applied. Paw withdrawal threshold (g) was recorded as the force that produced a 50% likelihood of withdrawal.

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