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Cell viability was detected using the MTT assay. Cells were cultured in triplicate in 96-well plates and treated with increasing concentrations of with berberine, erlotinib, cetuximab or the corresponding vehicles. After 48 hours, cell growth was measured by 0.5 mg/ml MTT. Cell viability was expressed as a percentage of control. The IC50 was calculated using Prism software version 4.03 (GraphPad Software Inc).
Copyright and License information: ©2016 Wang et al.
This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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