4.10. Cell Viability Assay

Cell viability assay was performed by the CellTiter-Glo Luminescent Cell Viability Assay Kit (Promega). For MI-503 treatment, T47D or MCF-7 cells were seeded in 96-well plate with 200 cells per well and treated with DMSO or MI-503 for 3 days. For circulated cells, 2000 cells after circulation were seeded in 50 μL of medium in 96-well plates. The assay reagent mixture was directly added into the cell medium at a 1:1 ratio for 15 min to lyse the cells, and the luminescent signal was detected using the Thermo Luminoskan Ascent Microplate Reader (Thermo Fisher Scientific).