2.4. HPLC Analysis of AEVM

HPLC analyses were done using a LaChrom Elite system (VWR-Hitachi, Radnor, Pennsylvania, USA) equipped with two L7100 pumps, a L7200 autosampler, a L2450 diode array detector (DAD), and EZ Chrom Elite software. Undigested and digested retreated samples were diluted five times and then chromatographed using a reversed phase Purospher^® Star C₈ endcapped column (125 × 4 mm, 5 μm particle size). A gradient elution was employed with a mobile phase composed of water containing 1% phosphoric acid (A) and MeCN (B). The gradient was set as follows: 0–5 min, 5% B; 5–30 min, 5–7% B; 30–45 min, 7–12% B; 45–50 min, 12–40% B. A flow rate of 1 mL/min, an injection volume of 50 μL, and a monitoring wavelength of 530 nm were selected.