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4.7. Immunohistochemistry (IHC)

WW Wei Wen
EH Ernest S. Han
TD Thanh H. Dellinger
LL Leander X. Lu
JW Jun Wu
RJ Richard Jove
JY John H. Yim
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Tumor samples were fixed in 10% formalin and embedded in paraffin. IHC was performed using VENTANA Ultra IHC automated stainer (VENTANA Medical Systems, Roche Diagnostics, Indianapolis, IN, USA) as described previously [66]. Slides were scanned with VENTANA iScan HT using VENTANA Image Viewer (VENTANA Medical Systems, Roche Diagnostics, Indianapolis, IN, USA). The images were taken at 20× magnification.

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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