- Home
- Protocols
-
Request a Protocol
Ask a
question
Rats were allowed to fast overnight and thereafter sacrifice by cervical dislocation once the experimental protocol was over. Heparinised vials were used to store the blood drawn via cardiac puncture. The blood was centrifuged at 1000g for 15 minutes immediately after collection. After sacrifice, the following day 1 and day 2 were utilized for analysis of blood and serum parameters. However, the tissues or tissue homogenates from the liver, pancreas and kidney were stored at –20 °C for further analysis.
Do you have any questions about this protocol?
Post your question to gather feedback from the community. We will also invite the authors of this article to respond.
Tips for asking effective questions
+ Description
Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.
Post a Question
