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2.5. Preparation of Mice Skin for In-vitro Skin Permeation and Deposition Studies
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Normal untreated mice were sacrificed after anesthesia. Full-thickness dorsal skin was excised and the hair on the dorsal area was removed using a depilatory cream (Nair, Church & Dwight Co., Inc., Ewing, NJ, USA). Subcutaneous tissue was surgically removed. The skin was washed with distilled water for immediate use [26]. For in-vitro skin permeation and deposition studies, mouse skin was randomly assigned to (a) formulation A group or (b) EtOH group.
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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
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