Inhibition of CD44

The P2 hAMSCs in the logarithmic growth phase were inoculated into 6-well plates at a dose of 2×10⁵ cells/well. The cells were divided into 4 experimental groups: The NC group; the positive drug group (PG); anti-CD44 antibody (cat. no. A3D8; GeneTex, Inc.) inhibition group NC + A3D8; and PG + A3D8. After 24 h of inoculation, the anti-CD44 antibody (2 µg/ml) was used to inhibit the expression of CD44 molecules in hAMSCs in the incubator at 37°C for 3 or 7 days. The medium was replaced with fresh medium after every 3 days. The expression of CD44 and chondrocyte-associated markers was detected on day 7.