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3.4. Photostability Measurements

AB Alexey M. Bogdanov
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The resin beads with immobilized proteins were placed in PBS and illuminated with an intense blue light (~1 W/cm2) using a fluorescence microscope. Changes in the fluorescence signal in the green channel were monitored during illumination. For wide-field fluorescence microscopy, a Leica AF6000 LX imaging system with Photometrics CoolSNAP HQ CCD camera was used. Fluorescence images were acquired using a 63 × 1.4NA oil-immersion objective and a standard filter set: GFP (excitation BP470/40, emission BP525/50). Photobleaching was monitored in time-lapse imaging in the green channel at low-light intensity combined with 5 s exposures to blue light of maximum intensity (GFP filter set). Images were acquired and quantified using Leica LAS AF software

Copyright and License information:  ©2020 by the authors.
Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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