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Luciferase reporter assay

YZ Yang Zhou
FL Fei Liu
QX Qinyang Xu
BY Bikang Yang
XL Xiao Li
SJ Shuheng Jiang
LH Lipeng Hu
XZ Xueli Zhang
LZ Lili Zhu
QL Qing Li
XZ Xiaolu Zhu
HS Hongfang Shao
MD Miao Dai
YS Yifei Shen
BN Bo Ni
SW Shuai Wang
ZZ Zhigang Zhang
YT Yincheng Teng
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Luciferase activity assays were carried out as described previously with some modifications, luciferase plasmids containing wild-type and 3 mutant PRKDC promoters were constructed in the pGL3B vectors with or without CDDP (1 μg/ml for 24 h). PRKDC promoters with plasmid CEBPD overexpressed SiHa cells were co-transfected with pGL3B. Luciferase activity was measured with a Dual-Glo luciferase assay kit (E290, E294, E2980, Promega, Madison, WI, USA). The sequences of primers the mutant constructs were listed in Supplementary Table 3.

Copyright and License information: The Author(s) ©2020
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