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Drug administration

MB Michela Bullone
AV Amandine Vargas
YE Yvonne Elce
JM James G. Martin
JL Jean-Pierre Lavoie
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Fluticasone/salmeterol combination and fluticasone alone were administered by means of an equine aerosol chamber (AeroHippus, Trudell Medical International), following the manufacturer instructions. Salmeterol powder was solubilized in sterile PBS at a concentration of 250 μg/ml, using Tween20 (Sigma-Aldrich) to facilitate solubility54. The dose of salmeterol administered (250 μg q8 h) was supported by previous data60 and by the results of preliminary tests we realized on asthmatic horses showing a significant bronchodilator effect persisting up to 6 hours post administration (Supplementary Fig. S5). Salmeterol was administered using a face-tight mask equipped with a mobile ultrasonic nebulizer (SaHoMa, NEBU-TEC International, Germany).

Copyright and License information: The Author(s) ©2017
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