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Palmitic acid preparation and treatment

NH Nirun V. Hewawasam
FL Fadel Lhaf
HT Henry A. Taylor
KV Katrina Viloria
AA Amazon Austin
AK Aileen King
PJ Peter Jones
LJ Lucy Jones
MT Mark D. Turner
NH Natasha J. Hill
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Palmitic acid solutions were prepared according to published protocols31. Briefly, 25.642 g of palmitic acid (PA) (Sigma P-500) was dissolved in 1 l of 0.1 M Sodium Hydroxide (NaOH) at 70 °C to obtain a stock 100 mM PA solution. In a 60 °C water bath, 5% (wt/vol) free fatty acid (FFA)-free bovine serum albumin (BSA) (Sigma A-6003) was prepared in serum-free RPMI medium. A stock solution of 5 mM PA/BSA complex was prepared by repeated vortexing followed by 60 °C incubation, then allowed to reach room temperature before sterile filtration (0.45 μm pore; Sigma N9020). This 5 mM PA/BSA solution was further diluted in RPMI-1640 to achieve the indicated final concentration. The solution was stored at − 20 °C for 3–4 weeks. Control cells were treated with a solution prepared identically except for the absence of PA.

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