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BrU-labeling and immunoprecipitation of newly synthesized RNA
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The BrU-labeling and immunoprecipitation of newly labeled RNA were carried out as for the mRNA decay assay except that the cells were harvested 45 min after addition of BrU to the cell culture medium. Furthermore, after binding of the RNA to the beads, the beads were washed once in 1x BrU-IP buffer, twice in 1x BrU-IP buffer supplemented with 0.01% Triton X-100 and twice in 1x BrU-IP buffer.
Copyright and License information: ©2020, Hollensen et al
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