2.6. Antioxidant Activity: DPPH Assay

The DPPH radical-scavenging activity was determined using the method proposed by [41] with minor modifications. Briefly, the DPPH methanolic solution 100 μM (1 mL) was added to polyphenol extract (0.2 mL), and the decrease in absorbance of the resulting solution was monitored at 517 nm after 10 min. The results were corrected for dilution and expressed as Trolox^® equivalent antioxidant capacity (TEAC, mmol Trolox^® equivalents Kg⁻¹ dry weight of plant). All determinations were performed in triplicate.