The plasma concentrations of CRH (CSB-E08038r), COR (CSB-E05112r), EPI (CSB-E08678r) and HSP70 (CSB-E08308r) were determined using ELISA (Cusabio Technology LLC). The optical density (OD value) was determined at 450 nm using an universal microplate reader (Bio-Rad Laboratories, Inc.). A standard curve was constructed for each component to determine their concentrations. The concentration values of CRH, COR and HSP70 are expressed in ng/ml and that of EPI in pg/ml.
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