Measurement of COR, EPI, CRH and HSP70 in the plasma

NZ Nan Zhao
LM Le Mu
XC Xiaoyu Chang
LZ Lingqing Zhu
YG Yao Geng
GL Guanghua Li
request Request a Protocol
ask Ask a question
Favorite

The plasma concentrations of CRH (CSB-E08038r), COR (CSB-E05112r), EPI (CSB-E08678r) and HSP70 (CSB-E08308r) were determined using ELISA (Cusabio Technology LLC). The optical density (OD value) was determined at 450 nm using an universal microplate reader (Bio-Rad Laboratories, Inc.). A standard curve was constructed for each component to determine their concentrations. The concentration values of CRH, COR and HSP70 are expressed in ng/ml and that of EPI in pg/ml.

Do you have any questions about this protocol?

Post your question to gather feedback from the community. We will also invite the authors of this article to respond.

0/150

tip Tips for asking effective questions

+ Description

Write a detailed description. Include all information that will help others answer your question including experimental processes, conditions, and relevant images.

post Post a Question
0 Q&A