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Infection of cells with Influenza A (IAV) PR8

GY Gracemary L. R. Yap
KS Karishma Sachaphibulkij
SF Sok Lin Foo
JC Jianzhou Cui
AF Anna-Marie Fairhurst
LL Lina H. K. Lim
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The day before IAV infection, A549 cells were seeded into tissue culture plates with complete F12K media. Before infection, media was aspirated and the monolayer is washed with 1X PBS and serum-free F12K media were added. Cells were infected with IAV PR8 virus with MOI 1 and placed in the incubator for 1 h. After 1 h, media was aspirated and replaced with complete F12K media.

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