Collection of saliva samples

Subjects performed an in-home saliva collection for three consecutive weekdays during the week following V1 and V4. One mL samples were collected in pre-labeled glass tubes at the following timepoints: 1) Immediately upon waking up, 2) 30 minutes after waking up, 3) between 2pm and 5pm, 4) between 7pm and 10pm. Participants were instructed to avoid eating/drinking, brushing teeth, or engaging in physical activity between the two morning sample collections. Tubes were stored in subject’s standard freezer until brought back to the lab, where they were stored at −80˚C until assay.