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Measurement of optical density using ImageJ
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The optical density of immunostaining was determined using the ImageJ software. As a first step, immunostaining images were acquired in the TIFF format. Then DAB-specific images were generated from initial images by color deconvolution of each image. The color devolution was performed using the set optical density vector for DAB and hematoxylin (H-DAB) in the color deconvolution plugin for ImageJ developed by Ruifrok et al [7]. Finally, the optical density of DAB in the area of interest (AOI) was determined using the ImageJ software.
Copyright and License information: ©2016 Yang et al
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
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