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Submerged cultivations in shake flask

AP Ashish A. Prabhu
DT Dominic J. Thomas
RL Rodrigo Ledesma-Amaro
GL Gary A. Leeke
AM Angel Medina
CV Carol Verheecke-Vaessen
FC Frederic Coulon
DA Deepti Agrawal
VK Vinod Kumar
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The fermentation medium had the following composition: (g/L) PG/CG/glucose, 20; xylose, 20; yeast nitrogen base (YNB), 1.7; NH4Cl, 1.5. The medium was prepared in 50 mM phosphate buffer. The initial pH was adjusted to 6.8 before inoculation by using 5 N NaOH. The submerged cultivations were carried out in 500 mL shake flasks containing 100 mL working volume. The flasks were inoculated with fresh inoculum at OD600 (optical density) of 0.1 and kept at 30 °C under constant shaking at 250 RPM on a rotary shaker (Excella 24, New Brunswick).

Copyright and License information: The Author(s) ©2020
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