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2.7. Transendothelial electrical resistance measurements
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Transendothelial electrical resistance (TEER) was measured following our established method (48) using an electrode compartment outside the vascular channels. Ag/AgCl electrodes were placed on either side of the HLMVEC or MLMVEC in the vascular and tissue compartments and connected to SynVivo Cell Resistance Analyzer (SynVivo Inc., Huntsville, AL). Impedance measurements were acquired at 10 kHz with a voltage of 10 mV.
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