4.5. LC–MS analysis

For metabolite profiling of hydroxynitrile glucosides, plant material was extracted by boiling in 85% methanol, essentially as described in Takos et al. (2011). Samples of extracts prepared at CIAT were dried down in 96‐wells microtiter plates and shipped to Copenhagen for analysis, where they were redissolved in 85% methanol (v/v) and filtered prior to analysis. Analytical LC–MS was performed using an Agilent 1100 Series LC (Agilent Technologies) coupled to a Bruker HCT‐Ultra ion trap mass spectrometer (Bruker Daltonics). A Zorbax SB‐C18 column (Agilent, 2.1 mm × 50 mm, 1.8 μM) was used with chromatography conditions as described previously (Takos et al., 2010). Compounds were localized in extracted ion chromatograms as sodium adduct ions: linamarin (m/z 270) and lotaustralin (m/z 284).