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1.2 × 104 cells per well were seeded the day before stimulation in 96 well plates. 50 µL of the Caspase-Glo 3/7 solution was added to each well. Caspase activity was measured according to manufacturer’s instructions (Caspase-Glo 3/7 Assay, Promega, Germany). The luminescence intensity was analyzed by a microplate reader Infinite M200pro (Tecan, Switzerland). The caspase activity of non-treated cells was taken as one relative unit (RU). Every condition was performed in duplicate.
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