Cell cytotoxicity assay

The CCK-8 assay was used to determine the SH-SY5Y cell viability. The SH-SY5Y cells were seeded into a 96-well culture plate at a density of 5 × 10³ cells/well and cultured for 24 hours. The cells were treated with different concentrations of sugammadex (50, 100, and 200 μg/mL) for 6 hours at 37°C, in an incubator. Subsequently, 10 μL of CCK-8 solution was added to each well, and the cells were cultured at 37°C for an additional 2 hours. The absorbance of the CCK-8 solution was measured at 450 nm using a microplate reader.