2.5. Quantitative Analysis of oxLDL Uptake

After 5 days of LDL treatment, collagen matrices were digested with 6 mg/mL of collagenase (Worthington Biochemical Corp., Lakewood, NJ, USA) prepared in cell culture media for 15 min at standard cell culture conditions to digest the collagen. Afterward, cells were analyzed using Attune NxT Flow Cytometer equipped with autosampler (Thermo Fisher Scientific, Inc.). Percentage of oxLDL uptake cells and mean fluorescent intensity were quantified using FlowJow Software (Build 10.5.3; BD Life Sciences). Experiments were performed in 5 replicates.