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4.4. Synthesis and Purification of maleimide-PEG12-maleimide Linker

MK Mateusz Adam Krzyscik
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Synthesis of maleimide-PEG12-maleimide (Mal-Ado6-Nε-(Mal)-L-Lys-CONH2) was performed on solid support according to the Fmoc-SPPS approach. A mixture of TFA/DMC/TIS/H2O (% v/v/v/v, 95/3/1/1) was used for the final cleavage from the resin. The crude linker was triply precipitated in ice-cold Et2O, purified by reversed-phase high-performance liquid chromatography (HPLC), and lyophilized.

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Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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